An optical fiber positioning method comprises the steps of: preparing an optical fiber, an optical fiber holder, and a positioning plate; fitting the optical fiber holder in the fitting hole and inserting the optical fiber into an optical fiber holding hole of the optical fiber holder and an optical fiber positioning hole of the optical fiber positioning plate; abutting the optical fiber on the abutting position of the optical fiber positioning hole in the state that the end of the optical fiber holder is fitted in the fitting hole and the optical fiber is inserted into an optical fiber holding hole of the optical fiber holder and an optical fiber positioning hole of the optical fiber positioning plate; and fixing the optical fiber to the optical fiber positioning plate in the state that the optical fiber is abutted on with the abutting position of an optical fiber positioning hole.