A light guide comb that produces a direct massage effect on the head of a user, and, moreover, uses light rays to promote cell activation of the head, cortical layer and acupuncture points, while simultaneously stimulating hair follicles to promote the health and growth of hair. The light guide comb includes an upper shell, a lower shell, a compartment, a light stimulation device and a light guide. A plurality of guide holes are defined in the upper shell to correspond to a plurality of guide pins configured on the light guide. Light rays produced by the light stimulation device disposed within the compartment are propagated through the plurality of guide pins and transmitted outward. A plurality of massage knobs are disposed on a comb head of the lower shell to provide a massage surface area greater than that of the plurality of guide pins.