A silver halide emulsion comprising silver halide grains covered with two dye layers in combination. The two dye layers comprises an inner dye layer adjoining to the silver halide grain, and containing at least one sensitizing dye capable of spectrally sensitizing silver halide, and an outer dye layer adjoining to the inner dye layer, and containing at least two dyes. The light absorption energy of the outer dye layer is equal to or higher than that of the inner dye layer, an energy-releasing wavelength of the outer dye layer overlaps with an energy-absorbing wavelength of the inner dye layer, the sensitizing dyes constituting the inner dye layer include an anion and/or betaine dyes, the dyes constituting the outer dye layer include an anion and cation dyes, and the anion dye is more than the cation dye in terms of an addition amount and/or an adsorption amount.

 
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> Compositions and methods for protein isolation

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