The present invention relates to a PPE family antigenic protein Rv2430c of SEQ ID No. 1; a set of three high antigenic index peptides of SEQ ID Nos. 2 to 4; an antigenic ORF Rv2430c of SEQ ID No. 5 and lastly a method of inducing immune response against Mycobacterium tuberculosis in a subject in need thereof, said method comprising step of: introducing PPE antigenic protein Rv2430c of SEQ ID No. 1 into the subject and inducing immune response against Mycobacterium tuberculosis.