The present invention provides a selection methodology of de-interlacing algorithm of dynamic image, comprising detecting hardware resource of player system. When hardware resource of player system corresponds to the executing condition of algorithm of line-segment de-interlacing, then selects algorithm of line-segment de-interlacing for de-interlacing. When hardware resource of player system doesn't correspond to the executing condition of algorithm of line-segment de-interlacing, then detects input image to see whether it contains information of P-frame or not. When input image contains information of P-frame, the player system selects algorithm of block-based de-interlacing for de-interlacing. Otherwise, the player system selects whether it takes complete frame as a process unit for deciding frame-based de-interlacing and field-based de-interlacing, and provides users with a higher-resolution dynamic image and a better elasticity of selection.