A hard disk drive fixing structure can accommodate a hard disk drive and can electrically connect with a computer to transmit information therebetween. The hard disk drive fixing structure has an accommodation casing; two rotary fixing members, which are corresponding to the fixing threaded holes of the hard disk drive, are pivotally installed to two lateral sides of the accommodation casing respectively; each rotary fixing member has at least one fixing protuberance; via the rotation of the rotary fixing members, the fixing protuberances can be respectively inserted into or withdrawn from the fixing threaded holes. Thereby, the hard disk drive can be fast assembled to/disassembled from the hard disk drive fixing structure of the present invention without any screwing/unscrewing activity but just via rotating the rotary fixing members.