To prepare high-performance specific gene-amplification primers for
detecting, quantitatively determining or identifying Vibrio
parahaeinolyticus having low possibility of mis-identification and
practically sufficient amplification efficiency and amplification
specificity.We have determined the nucleotide sequences of rpoD genes
encoding RNA polymerase .sigma.70 factor of type strains of the genus
Vibrio and of stock strains of Vibrio parahaemolyticus (strains
containing and those not containing toxin gene); elucidated the
phylogenetic relation, and identified nucleotides which are
characteristic in Vibrio parahaemolyticus, thereby enabling the design of
probes containing them and having high specificity, and primers for gene
amplification having high specificity and excellent amplification
efficiency.