An acupuncture needle guide assembly includes an acupuncture needle with a needle handle and a needle part, a guiding tube accommodating the acupuncture needle before use and providing guidance for setting the acupuncture needle, a holding tube being arranged in the guiding tube and providing a cover for the needle part of the acupuncture needle during setting thereof, and a detachable connector fixing the holding tube between the needle handle of the acupuncture needle and an inner wall of the guiding tube.