A system for testing a cable network during normal operation interleaves test signals with live data traffic. Installed cable modems are used to generate the test signals. During live communication, such modems send upstream data at allotted time periods. Periodically, there are time slots when no modem is transmitting on the network. During these times, specific modems may be directed to transmit specific test signals. A controller such as a cable modem termination system directs specific modems to transmit signals at defined frequencies and powers during specific time slots. In this manner, the system can monitor the frequency response of the cable network at various locations, and thereby determine "on-the-fly" when maintenance or modification is required.