The present invention describes a method of selective peptide isolation
for the identification and quantitative analysis of proteins in complex
mixture. The method comprises the selective isolation from every protein
of those peptides that neither contain arginine nor histidine (NHNR
peptides), and the determination of the relative concentrations of one or
several proteins in different samples from the ratio between the areas of
the estimated theoretical spectra for the NHNR peptides labeled with
different isotopes in each sample. The determination of the relative
concentration of proteins is valid for any type of isotopic label of the
NHNR peptides. The method avoids the separation and purification of the
proteins present in a complex mixture, and the analysis of all peptides
generated from the enzymatic digest of the samples. The method is
applicable to the identification of proteins with vacunal, therapeutic
and diagnostic aims.