Oligonucleotide primers and polynucleotide probes provide selective
amplification and detection of Id genetic sequences and are selected both
(1) to provide the desired specificity so that they amplify only the
specific Id type to which they are targeted; and (2) to introduce
terminal restriction endonuclease cleavage sites into the amplicon that
facilitate the incorporation of the amplicon into plasmid-based vectors.
Detection of Id genetic sequences can be carried out using the
labeled-amplicon by reamplifying the amplicon with the primers in the
presence of labeled, for example radiolabeled, deoxynucleotide
triphosphates. The probes of the invention correspond in sequence to the
amplicons produced using the primers of the invention, after cleavage at
the restriction endonuclease sites.