The present invention provides a kind of HBV-antigen bound to heat shock proteins (hsps) which comprises core antigen, surface antigen and polymerase antigen. The present invention also provides a complex of HBV antigen bound to heat shock protein gp96 and hsp78, as well as a method for preparing the complex. The complex includes a complex of gp96 and hsp78 non-covalently bound to antigenic polypeptide, as well as a fusion protein of both which results from covalent binding. Such complex can be used to prepare therapeutic vaccine for treating hepatitis B and primary hepatocellular carcinoma.