An automatic gain control device for an electronic endoscope is provided and comprises a controllable gain amplifier, an A/D converter, a histogram circuit, and a CPU. The controllable gain amplifier amplifies image signals from an imaging device. Amplified image signals from the controllable gain amplifier are input to the A/D converter. A histogram of the amplified image signals from the controllable gain amplifier is produced by the histogram circuit when an image taken in a white-balance test accessory is taken. By using the CPU, the gain of the controllable gain amplifier is adjusted in accordance with the determination of whether a saturated pixel exists for signals in the tolerance range of the A/D converter, so that a linear region of the image signals from the controllable gain amplifier substantially coincides with the tolerance range.