Immunological assays for several biological markers for thyroid disorders
in a biological sample are performed in a single test with a combination
of sandwich-type, sequential competitive, and serological assays by the
use of particles classified into groups that are distinguishable by flow
cytometry, one group for the assay of each marker. Each group of
particles is coated with a different immunological binding member, and
coating densities, co-coating materials, and special buffer solutions are
used to adjust for differences in the sensitivities and dynamic ranges of
each of the markers in the typical sample.