Processes for the isolation of low-molecular weight aminoglycan compound
of formula I from a hitherto unexploited natural source of waste egg
shells which is made up of alternating glucuronic acid and N-acetyl
glucosamine units comprising the steps of: (a) pre-preparation of the
waste egg-shells for extraction of the embryonic low molecular weight
aminoglycan compound of formula I using a polar organic solvent in water;
(b) extraction of the low molecular weight aminoglycan compound of
formula I as its soluble salt using an aqueous polar salt solution; (c)
isolation of a purified low molecular weight aminoglycan compound of
formula I by gel formation out of the aqueous salt mixture by using a
polar organic solvent followed by filtration or centrifuging; (d)
stabilization of the isolated aminoglycan extract by sequential
introduction of organic oils into a semi-dried gel to form ordered sheets
of aminoglycan compound of formula I with no visible cross-linking of the
carbohydrate polymer.