Disclosed are oral hygiene regimens comprising a plurality of steps involving the use of two or more oral care products containing actives effective to provide one or more benefits including cleaning, antimicrobial, antiplaque, anticaries, anti-calculus, anti-demineralizing, anti-erosion, antisensitivity/desensitizing, whitening, surface conditioning, polishing, and pH-balancing, wherein the plurality of steps are conducted and sequenced for maximizing delivery of oral care actives to target surfaces of a subject's oral cavity thereby achieving optimum oral health and hygiene benefits. In one aspect, a regimen comprises at least once daily of each of the following steps: (a) applying an antimicrobial dentifrice to oral cavity surfaces, and (b) rinsing the oral cavity with an antimicrobial mouthrinse,wherein at least one of steps (a) and (b) is conducted prior to retiring at night. Preferably the regimen comprises as step (c) treating interproximal, gingival and subgingival areas of the oral cavity using an interproximal device selected from dental floss, floss pick, dental tape, and proxy brush, wherein step (c) is conducted immediately before or immediately after one or both of steps (a) and (b).