A method for producing isolated limonoid glucoside compounds is disclosed which includes extracting a quantity of defatted citrus seed powder with a solvent, filtering the resulting extract to yield a particle free extract and concentrating the particle free extract to yield a concentrate. The concentrate is loaded onto an ion exchange column that is coupled to an adsorption column. The ion exchange column is washed with deionized water and the eluate from the ion exchange column is passed through the adsorption column. The adsorption column is then eluted using a series of mobile phases in which composition of each successive mobile phase is graduated. A series of eluate fractions are collected from the adsorption column and concentrated to produce isolated crystals of limonin glucoside, deacetyl nomilinic acid glucoside and isoobacunoic acid glucoside.