There is provided an isolated polynucleotide sequence useful as a promoter
in plant root tissue. The sequence is derived from the promoter for the
Western white pine gene PR10. The sequence comprises at least 45
nucleotidcs, and has at least two Dof elements which individually may be
in a forward or inverted orientation. Each Dof element has a sequence
NNNWAAAGNNN, wherein N is A, T, G, or, C, and, W is A or T. The Dof
elements are separated from each other by between 10 and 20 nucleotides.
The sequence also has a TATA box located between 15 and 25 nucleotides in
the 3' direction of the closest Dof element. In some instances, the
polynucleotide has one Dof element with the sequence NNNWAAAGNNN and
another with the sequence NNNCTTTWNNN. In some instances the
polynucleotide sequence is one where the 5' Dof element sequence is
CTCTCTTTAAT, and another Dof element (which may be the next Dof element)
sequence is TTTAAAGGTT and the TATA box sequence is TACAATAAATA or
TATAWAWA, wherein W is A or T. Also provided are sequences useful in
modulating gene expression in stem and leaf tissue.