The present invention includes modified phytochrome A (PHYA) nucleic acid
molecules in which Pr absorption spectra have been shifted to longer
wavelength (i.e. bathochromism or red-shift). The plants with the
bathochromic phytochromes are expected to respond to canopy and shade
conditions for growth and development with greater efficiency than the
plants with wild-type phytochrome (i.e. suppression of shade avoidance
reactions in plants). Since the shade avoidance reactions in plants
induce a rapid and dramatic increase in the extension growth of stems and
petioles at the expense of leaf growth, storage organ production, and
reproductive development, it causes significant losses of crop yields.
Thus, the bathochromic phytochromes that utilize the shade light
efficiently would suppress the shade avoidance reactions in plants,
giving plants the tolerance to shade. In this invention, several
bathochromic phytochromes were generated by site-directed mutagenesis in
the region of bilin lyase domain in plant PHYA, and their ability to
suppress the shade avoidance reactions were examined by transforming the
bathochromic phytochromes into PHYA deficient Arabidopsis thaliana
(ecotype col-0). The transgenic plants with the bathochromic phytochromes
showed significantly increased shade tolerance compared to wild-type
plants and transgenic plants with wild-type phytochromes. Therefore, the
present invention can be utilized to suppress plants' shade avoidance
that is one of major causes to induce crop-yield losses, and ultimately
to generate shade tolerant plants with higher yields. The invention also
includes plants having at least one cell expressing the modified PHYA,
vectors comprising at least one portion of the modified PHYA nucleic
acids, and methods using such vectors for producing plants with shade
tolerance.