An operation training simulation system for a computer numerical control (CNC) machine includes a physical control device for inputting control and operation commands, a software simulator connected with the physical control device, a transmission apparatus connecting the physical control device with the software simulator and a simulation display electrically connected to the software simulator. The software simulator generates machining and operation simulations based on the inputted control and operation conditions through the physical control device, and the simulation display visualizes the machining and operation simulations generated by the software simulator. Therefore, using the operation training simulation system for CNC machine not only eliminates the consumptive materials cost but also solve the personal injury problem. Consequently, the learning efficiency of the trainees and the familiarization to machine are raised in this way.