A change in viral tropism occurs in many HIV positive individuals over
time and can be indicated by a shift in coreceptor use from CCR5 to
CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate
with increased disease progression. In patients undergoing HAART, the
predominant populations of virus can be shifted back to CCR5-mediated
entry after the CXCR4-specific strains have emerged. The present
invention relates to a diagnostic method to monitor coreceptor use in the
treatment of human immunodeficiency virus (HIV) infection. The present
invention further relates to a diagnostic method applied to HIV-positive
individuals undergoing HAART to monitor the suppression of CXCR4 specific
strains. The diagnostic methods can be used to assist in selecting
antiretroviral therapy and to improve predictions of disease prognosis
over time.