The present invention provides a fluid sampling device comprising a port insert, a plurality of flexible conduits, and a plurality of sample containers. The port insert comprises a body having a plurality of shafts therethrough and sample gating means for individually opening and closing any of said shafts to enable the flow of fluid therethrough. The flexible conduits (e.g., flexible tubing) are equal in number to the elongate member, with each flexible conduit connected to or otherwise in fluid communication with an individual shaft. Similarly, the sample containers (e.g., flexible bags) are equal in number to the conduits, with each sample container connected to an individual conduit opposite the connection to the shaft. A specific configuration for the port insert, as well as kit containing sterilized components of the fluid sampling device, are also described.