Discloses is a cartridge-type detection apparatus which comprises a detection cartridge having a passage for passing a sample liquid containing an target substance, and a processing unit adapted to be loaded with the detection cartridge so as to produce information about the target substance contained in the sample liquid passed through the detection cartridge. The detection cartridge includes an storing section for temporarily storing the target substance, a liquid passage routed through the storing section, and a plurality of ports in liquid communicate with the liquid passage. The detection cartridge is provided with a part or entirety of a detection mechanism on a downstream side relative to the storing section. The processing unit includes a liquid feed pump and a line switching valve mechanism adapted to switchingly provide liquid communication between the liquid feed pump and a selected one of the plurality of ports of the detection cartridge. The valve mechanism is operable to switch between a first passage connection mode for allowing the sample liquid supplied into the detection cartridge to be passed through the storing section and then discharged out of the detection cartridge, and a second passage connection mode for allowing a reagent to be supplied from one of the plurality of ports to the storing section of the detection cartridge by an action of the liquid feed pump, and allowing the reagent passed across the storing section to be discharged out of the detection cartridge from one of the remaining ports.