The present invention provides methods of refolding mammalian
glycosyltransferases that have been produced in bacterial cells, and
methods to use such refolded glycosyltransferases, including
glycosyltransferase mutants that have enhanced ability to be refolded.
The invention also provides methods of refolding more than one
glycosyltransferase in a single vessel, methods to use such refolded
glycosyltransferases, and reaction mixtures comprising the refolded
glycosyltransferases.