Described is a dark-field imaging device for the spatially resolved dark-field imaging of a sample (60), especially a fluorescent sample, comprising, for the illumination of the sample with excitation light, an illumination assembly (20) comprising a grid (22) of individually addressable light sources (24), wherein the lines connecting the light sources and the sample define a region (64) with the optical path of the direct excitation light and a region (66) with the optical path of the specular reflected excitation light, and a detection assembly (30) for the detection, with radiation-receiving elements (34), of the radiation emitted by the sample (60) as an optical response to the illumination, wherein the radiation-receiving elements (34) of the detection assembly (30) are disposed outside the region (64) with the optical path of the direct excitation light and the region (66) with the optical path of the specular reflected excitation light.