To provide a new technique by which efficient transfection is ensured in
delivering a target gene into a cell, disclosed is a nucleic acid
construct for nuclear import, which comprises a ternary complex
consisting of a nucleic acid substance containing a gene to be delivered
into the nucleus of a cell, an importin protein (for example,
importin-.beta.) capable of passing through the nuclear pore and involved
in the nuclear transport, and a binding substance (for example,
polyethyleneimine) bound to both of the nucleic acid substance and the
importin protein. Nucleic acid transport from outside of a cell into the
cell nucleus can be particularly promoted by administering the nucleic
acid construct bound to a cell membrane receptor binding factor and/or a
membrane fusing substance, or administering the nucleic acid construct
encapsulated in a non-viral vector (for example, Sendai virus envelope)
having cell membrane permeability and membrane fusing properties.