A method is provided for generating sustained totipotent tissue cultures
of a plant of the Class Monocotyledonae, and for micropropagating such
plant in vitro, wherein immature inflorescence are cultivated to produce
totipotent tissue which is suitable for sustained maintenance and
propagation. Greening of the tissue can be induced under light and the
multishoot culture can multiply by microtillering. Foreign genes can be
introduced into the tissue if desired, and the transgenic plants can be
used in phytoremediation technologies in the field and in phytoreactors
independently of seasons.