The present invention relates to the detection of analyte(s) of interest in a test sample using a rupturable microcapsule biosensor. In general, the microcapsule biosensor includes a microcapsule comprising a shell encapsulating a detectable agent and further includes a probe that is joined to the shell of the microcapsule. The present invention contemplates generally using the microcapsule biosensor to determine if a test sample contains an analyte of interest by exposing the microcapsule biosensor to the test sample and allowing the probe to potentially bind to the analyte. External force is applied to the microcapsule to rupture the shell releasing the detectable agent and thereby indicating the presence of the analyte of interest in the test sample.