A method for testing an image sensor for photoresponse uniformity, the method includes the steps of providing a light source having inherent non-uniform light intensity characteristics; providing a light sensing device for measuring an intensity response of the light source on a predetermined image plane; determining an area on the image plane corresponding to an area for the image sensor; measuring pre-selected points of the determined area by the light sensing device for measuring incident light intensity from the light source on the image plane; interpolating non-measured points on the image plane representing an estimate of the non-uniformity of the light source at the non-measured points; inverting the interpolated image to represent the inverse of the light source non-uniformity; and applying the inverted image to the response of the image sensor which compensates for light source non-uniformity and enables the ability to quantify accurately the non-uniformity of the image sensor.