The present invention provides a method of efficiently and stably
producing LCPUFA-PL. Specifically, the invention provides a method for
producing phospholipids that contain LCPUFA as a constituent (LCPUFA-PL),
wherein lipid producing cells producing lipids that contain long-chain
polyunsaturated fatty acids (LCPUFA) are used as a starting material, the
method including a PL extracting step of extracting phospholipids (PL)
from defatted cells obtained by extracting triglyceride (TG)-containing
oil or fat from the lipid producing cells.