A biological tissue sheet which is expected as exerting a favorable
therapeutic effect and a high safety in transplantation. The biological
tissue sheet formed by (a) preparing in vivo-derived cells; (b) sowing
the in vivo-derived cells on amniotic membrane; and (c) culturing and
proliferating the in vivo-derived cells in the absence of any xenogeneic
animal cells. As the cells of a biological origin, for example, cells
originating in corneal epithelium, conjunctival epithelium, skin
epidermis, hair follicle epithelium, oral mucosa, respiratory tract
mucosa, or intestinal tract mucosa.