The present invention provides a novel fusion protein suitable to be expressed high effectively and the production method thereof. The structure of the fusion protein is A-C1-K-(B-C2-K)n-D, wherein A is the upstream peptide; each of C1 and C2 is independently short peptides having 20-40 amino acids in length and containing no Lys in sequence; K is Lys; B is a linker; D is a downstream peptide; n is an integer of 3-30. The fusion protein is able to express high effectively and stably in host cells. The purification and enzymatic cleavage technology of the expression product is simple, which is able to produce a short peptide high effectively, and has very high value of industrialization.