A microfluidic detection device provides reduced dispersion of axial
concentration gradients in a flowing sample. The microfluidic detection
device includes a cell body and a flow path through the cell body. The
flow path has an inlet segment, an outlet segment, and a central segment,
which forms a detection cell. The central segment is located between and
at an angle with both the inlet segment and the outlet segment. The
central segment has a first junction with the inlet segment and a second
junction with the outlet segment. The cell body contains two arms that
can transmit light to and from the detection cell. At least a portion of
a first arm is located in the first junction and at least a portion of a
second arm is located in the second junction. The portions of the arms
located in the junctions are situated so that fluid entering or exiting
the central segment of the flow path flows around the outer surface of
one of the portions. By ensuring that the flow velocity is high near the
walls both at the beginning and at the end of the conduit, the
configuration serves to counteract dispersion caused by the normal
parabolic velocity profile of flow through a cylindrical conduit, where
the fluid velocity is highest at the center. In addition, the
configuration promotes efficient sweeping of the entire volume between
the two arms. A method for manufacturing the microfluidic detection
device is also provided.