Four zebrafish gene promoters, which are skin specific, muscle specific,
skeletal muscle specific and ubiquitously expressed respectively, were
isolated and ligated to the 5' end of the EGFP gene. When the resulting
chimeric gene constructs were introduced into zebrafish, the transgenic
zebrafish emit green fluorescence under a blue light or ultraviolet light
according to the specificity of the promoters used. Thus, new varieties
of ornamental fish of different fluorescence patterns, e.g., skin
fluorescence, muscle fluorescence, skeletal muscle-specific and/or
ubiquitous fluorescence, are developed.