Methods for expressing a functional heteromeric taste receptor that
responds to sweet taste stimuli are provided. These methods comprise the
co-expression of T1R2 and T1R3 nucleic acid sequences in a host cell that
desirably further expresses a G protein that couples therewith, e.g.,
G.sub..alpha.15, G.sub..alpha.16 or gustducin. In preferred embodiments,
the host cells will be mammalian cells or Xenopus oocytes. These nucleic
acid sequences are expressed constitutively or under inducible
conditions. In preferred embodiments the expression methods will use
HEK-293 cells that also stably express G.sub..alpha.15. These methods
give rise to heteromeric receptors and compositions containing that are
useful in assays for identifying novel sweeteners and sweetness
modulators.