The invention is further directed to producing a cleaned, disinfected, and
devitalized cartilage graft by optionally cleaning and disinfecting the
cartilage graft; treating the cartilage graft in a pretreatment solution;
treating the cartilage graft in an extracting solution; washing the
extracted cartilage graft with a rinsing solution; and subsequently
soaking the devitalized cartilage graft in a storage solution. The
devitalized cartilage graft is essentially free from metabolically viable
and/or reproductively viable cells and the rinsing solution is hypotonic
solution or isotonic solution. The present invention is further directed
to a cleaned, disinfected, and devitalized cartilage graft and a process
for cleaning, disinfecting, and devitalizing cartilage grafts. The
invention also relates to a process for repairing a cartilage defect and
implantation of a cartilage graft into a human or animal by crafting the
cartilage matrix into individual grafts, disinfecting and cleaning the
cartilage graft, applying a pretreatment solution to the cartilage graft,
removing cellular debris using an extracting solution to produce a
devitalized cartilage graft, implanting the cartilage graft into the
cartilage defect with or without an insertion device, and sealing the
implanted cartilage graft with recipient tissue. The devitalized
cartilage graft is optionally recellularized in vitro, in vivo, or in
situ with viable cells to render the tissue vital before or after the
implantation. The devitalized cartilage graft is also optionally stored
between the removing cellular debris and the recellularizing steps.