The invention relates to high-level production of pHBA in green plants
using a unique expression cassette. The latter comprises a chorismate
pyruvate lyase (CPL) coding sequence operably linked to a suitable
promoter capable of driving protein expression in higher plants.
Additionally, the CPL cassette comprises a sequence encoding a
chloroplast transit peptide, its natural cleavage site, and a small
portion of the transit peptide donor protein fused to the N-terminus of
CPL. The chloroplast targeting sequence targets the foreign protein to
the chloroplast compartment and aids in its uptake into the organelle.
The cleavage site is unique to the transit peptide, and cleavage of the
chimeric protein encoded by the cassette at this site releases a novel
polypeptide that has full enzyme activity, comprising the mature CPL
enzyme and a small portion of the transit peptide donor.