Provided is separation media comprising: a base mixture having an adsorptive organic component and an inorganic component; an anti-microbial component comprising a quaternary ammonium salt containing an epoxide group; and a covalent bond directly between the quaternary ammonium salt containing an epoxide group and the inorganic component. The covalent bond is, for example, between the epoxide group and a hydroxyl group of the inorganic component. The quaternary ammonium salt containing the epoxide group can be poly(methyldiallylamine epichlorohydrin). Further, the quaternary ammonium salt containing the epoxide group can have the formula according to I: wherein n is in the range of 5 to 24. The inorganic component can be diatomaceous earth, and the adsorptive organic component can be activated carbon.