The present invention relates to a RUNX3 gene showing anti-tumor activity
which is essentially involved in TGF-.beta. dependent-programmed cell
death (apoptosis) and use thereof. In addition, the present invention
finds that the RUNX3 gene expression is suppressed in the various gastric
cancer and lung cancer cell lines. The suppression of the RUNX3 gene
expression is due to hyper-methylation of CpG island located around RUNX3
exon (1). The RUNX3 gene and its gene product of the present invention
can be used effectively for the development of anti-cancer agents. CpG
island around RUNX3 exon (1) could also be used not only for the
development of anti-cancer agents which regulate the abnormal DNA
methylation and there by induce RUNX3 expression but also for the
development of methods for cancer diagnosis by measuring the abnormal DNA
methylation.