In an apparatus and method for measuring parameters related to retardance
and slow axis azimuth in sample specimens, a sample is illuminated by
circularly polarized monochromatic light which is then analyzed by an
elliptical analyzer at different settings. In another embodiment, light
conditioned by an elliptical polarizer at various settings illuminates a
specimen and the beam exiting the sample is analyzed by a circular
analyzer. Background images obtained with selected settings of the
elliptical analyzer/polarizer, but without the sample present. Algorithms
are described which employ two specimen images with elliptical settings
and three or two background images; or which employ three specimen images
with elliptical settings; or which employ four specimen images with
elliptical settings without an extinction setting; or which employ five
specimen images with four elliptical settings and one extinction setting.
These algorithms allow one to variously optimize measurements for speed,
sensitivity, and accuracy.