A method for seamless inter-frequency hard handover in a radio communication system is disclosed. The method for seamless inter-frequency hard handover includes the steps of: a) at a mobile station, blocking a first uplink carrier frequency used for communication, transmitting a direct sequence spread preamable signal through a second uplink carrier frequency for a short time, and continuously performing the communication through the first uplink carrier frequency: and h) at a target base transciever station, acquiring an uplink synchronization of a mobile station based on the preamble before performing handover.