In one embodiment, an image sensor module having an auto-aligned lens includes: a substrate on which an image sensor chip is mounted; a housing which has an opening to expose an upper surface of the image sensor chip, and which is attached onto the substrate; a lens holder which extends the opening vertically and upwardly; and a lens unit incorporating spacers which is fixed to an inner sidewall of the lens holder and is aligned automatically. Friction-caused particles are not produced, and focal alignment is readily automated.