The apparatus and method for quickly measuring the hemoglobin content of a
sample of blood uses a light reflectance measurement of the hemoglobin
sample lysed in a membrane. The reflection is inversely proportional to
hemoglobin concentration. A blood sample is loaded on to a nylon mesh and
dispersed within a nylon membrane, both of which have been treated with a
hemolyzing agent and surfactant, and air-dried. As soon as the blood
sample contacts the reagent on the membrane, the red blood cells are
lysed and the hemoglobin molecules are released and dispersed into the
membrane by the action of surfactant. The hemoglobin apparatus emits
light at 522 nm to the reflective surface of the membrane. The intensity
of the reflected light is measured by a detector in less than 29 seconds.
The intensity of the reflected light is converted to hemoglobin
concentration, g/dL, or mmol/L by an interpolative comparison with stored
reflection data indicative of known hemoglobin contents.