Pretreatment of residual food removal and bacteria dyeing, and measurement
of the number of bacteria using the fluorescence flow cytometry method,
are stably performed by easy operations.
A measurement chip 10 includes: a specimen container 151; a reaction
container 170 for mixing a specimen and a dyeing reagent into a liquid
mixture; a bacteria detection portion 18 being irradiated with excitation
light; a detection liquid waste container 163 into which liquid mixture
which has passed the bacteria detection portion enters; a solution flow
path 157 which connects the specimen container, the reaction container
and the bacteria detection portion, and ventilation ports 1631 through
1633 which are connected to the specimen container, the reaction
container and the detection liquid waste container, through a air flow
path and is connected to a chip connecting tube. Bacteria are detected in
the bacteria detection portion 18 by switching of the states of the
specimen container, the reaction container, and the detection liquid
waste container between a sealed state and a state open to the
atmospheric pressure, by moving the specimen to the reaction container
170 to mix and stir the specimen and the dyeing reagent, and by moving of
the liquid mixture to the detection liquid waste container 163.