A bio-artificial organ comprises a substrate comprising a roll of a substrate material, and a plurality of cells adhered to the substrate, the roll being formed from a sheet rolled to form a plurality of layers that include spacers and spaced openings such that at least a first set of parallel chambers is formed when the roll is formed, the chambers being manifolded to a first inlet and a first outlet. The bio-artificial organ may further include at least a second chamber, the second chamber being isolated from the first set of chambers by at least a cell barrier. A method for assembling a bio-artificial organ comprises a) providing a substrate for cell culture capable of forming a roll, the substrate having a surface, b) patterning the surface of the substrate, c) seeding cells onto the substrate, and d) reeling the substrate into a cylindrical roll.