A seal structure of separator for fuel cell comprising:two separators disposed opposite to one another, which have electrode portions, manifold portions circulating fuel gas fed to said electrode portions, passage grooves feeding fuel gas between said electrode portion and said manifold portion, and hole portions opposite to the manifold portions of a facing separator;a seal body, which is provided integrally on the separators and has a first seal portion to seal an area surrounding the electrode, the passage groove and the manifold portion, and a second seal portion to independently seal the hole portion;an electrolyte membrane, sandwiched between the two separators, having a size enough to include the electrode portion, the manifold portion and the hole portion, and having a shape with a through-hole on the manifold portion and at a portion of the hole portion; anda gasket surrounding the manifold portion of the separators and straddling the passage groove to enable fuel gas to be distributed.