The present invention relates to protein engineering, and concerns
especially family G/11 xylanases, and genes encoding said enzymes. In
specific, the invention concerns Trichoderma reesei XYNII gene, which
codes for endo-1,4-.beta.-xylanase (EC 3.2.1.8). The invention describes
how site-directed mutagenesis can be used to improve the properties of an
enzyme to match the industrial conditions where it is used. Protein
engineering can be used to improve thermoactivity and thermostability of
xylanases, as well as to broaden their pH range.