The present invention relates to a process of obtaining recombinant
lambdoid bacteriophage with high density display of functional peptides
and proteins on surface of said phage comprising of: constructing a donor
plasmid having a nucleotide sequence that defines the elements for
replication of the vector in bacteria, a selectable marker, a nucleotide
sequence flanked by two non-compatible recombination sequences, and an
inducible cistron for expression of a capsid protein and a fusion
protein; constructing a recipient phage having a nucleotide sequence that
defines the lambdoid elements for replication and packaging of the vector
into an assembled bacteriophage and encodes an inducible cistron for
expression of a selectable marker flanked by two non-compatible
recombination sequences; transferring the said donor plasmid to said
recipient plasmid to obtain cointegrates; growing said cointegrates in
selective liquid medium; harvesting phages displaying protein encoded by
the foreign DNA encapsulated in said harvested phage particle.