An optical fiber connection jig which is small in size, lightweight and excellent in portability, simple in structure, can be produced at a low cost, and enables an optical fiber-connecting operation at the field to be carried out easily and positively. The optical fiber connection jig includes a jig body having wedge pieces which are fitted respectively into wedge insertion slits in a splice member by a grasping operation for a pair of left and right grasp frames opposed to each other with the splice member disposed therebetween, thereby forcibly expanding an optical fiber holding groove, and a fiber insertion guide which is mounted on the jig body, and guides a coated optical fiber generally onto a center axis of the optical fiber holding groove by a coated fiber guide groove extending in a direction of the center axis Y1 of the optical fiber holding groove.