The invention relates to methods of processing amniotic membrane to
generate a substantially `growth factor free` membrane (GFF-membrane),
and to methods of processing GFF-membrane to generate membrane enriched
with specific and quantified levels of growth factors or other desirable
membrane enriching molecules or compounds (E-membrane). The invention
extends to GFF-membrane per se and E-membrane enriched with specific and
quantified membrane-enriching compounds per se. The method also includes
first and second medical uses of GFF-membrane and E-membrane. The method
also extends to clinical uses of the amniotic membrane spongy layer or
components thereof.